Pumps are one of the most common tools enabling liquids to be moved, delivered, pushed through a separation column, supplied as a dose or transferred from one container to another. Several different pumping technologies are available to choose from, depending on your application or problem. As you might expect - a
pump designed to pump crude oil through a pipeline has very different requirements compared to a pump designed to deliver a constant stream of medication into a
patient’s vein. As such, the term ‘flow’ can be interpreted very differently, depending on the context.
Published in International LabMate – July 2022
Peristaltic pumps find wide application in modern labs, they are considered inexpensive, easy to use and to maintain and, most important of all, are extremely reliable and flexible. The range of application is huge, from simple transfer of a liquid from one container to another in HPLC and Ion chromatographs, to more complex usage as dispensing tools for reagents in flow chemistry.
Published in MicroBioz India – June 2022
With peristaltic pumps, several flow paths can be operated simultaneously. However, many parameters influence the actual flow rate in each channel. Accurate determination of the individual flow rate in the individual channels is crucial for reproducible results.
Published in Lab Worldwide – February 2022
Liquid Chromatography of any kind - HPLC, UHPLC, GPC/SEC, Ion Chromatography or FPLC, is based upon the specific characteristics of the utilised separation column to provide a suitable environment for a particular application or investigation. As such, creating an optimised separation is often considered to be the core of an individual liquid chromatography system. Separation alone, however, does not supply answers, it just creates the correct preconditions whereby chromatography detectors are able to reveal the 'secrets' of your sample. Read on
This article provides an overview about the detectors most commonly used in GPC/SEC applications, explains differencies within each detector class, provides inforamtions about pro's and con's of each detector and also explores combination of detectors for the most useful hyphenated techniques in GPC/SEC. Read on
The specific refractive index increment (dn/dc) is a fundamental parameter for the determination of molecular weight with Static Light Scattering. Its importance will become clear by observation of the Debye's constant in the SLS equation, where the dn/dc is a squared term. As such, any error in the specific refractive index increment will strongly effect the molecular weight results. This is true for both cases, when SLS is used in batch mode, like in case of a goniometer system, or in flow mode like in case of a MALS Detector in GPC/SEC. Read on
Why are Testa Analytical involved in polymer characterization and particle sizing? In the eyes of Testa Analytical polymer characterization in terms of chromatography and particle sizing using DLS are just two ways of looking at the same subject. We look at the same material from two different points of views which in turn gives us a better understanding of the whole material which is exactly what Testa Analytical strives to provide the solution for. Read on
TESTA Analytical Solutions eK (Berlin, Germany), a five-year old developer of size exclusion chromatography (SEC) instruments and related detectors and software, and distributor of third-party dynamic light scattering (DLS) and Zeta Potential systems, is aiming to build on its success in the European OEM market to provide “worldwide operating clients with application-optimised versions of our growing portfolio of proprietary instruments and detectors,” Carlo Dessy, the company’s founder, owner and CEO, told Instrument News. Read on
Design Considerations for Refractive Index Detectors - different instrument techniques, different goals
The detection technique of choice for HPLC separation of non-UV active substances, such as saccharides, is a Differential Refractive Index (DRI) detector. In most cases, the required analysis is purely quantitative and based on comparison of the area below the sample peak with the area below the peak of a run performed with the same compound at a known concentration. Click here to download
High Performance Liquid Chromatography (HPLC) is nowadays one of the most widely used techniques in analytical chemistry. The range of applications soluble using HPLC covers a vast range of sample types, concentrations, and compositions. This extreme flexibility is achieved by using specific columns carefully designed for each particular investigation. Click here to download
GPC (Gel Permeation Chromatography) or SEC (Size-Exclusion-Chromatography is widely acknowledged as one of the most useful characterization methods in polymer science. The technique offers the possibility to determine molecular weight distribution and its averages, to provide a complete picture of the polymer sample under investigation. Click here to download
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