The specific refractive index increment (dn/dc) is a fundamental parameter for the determination of molecular weight with Static Light Scattering. Its importance will become clear by observation of the Debye's constant in the SLS equation, where the dn/dc is a squared term. As such, any error in the specific refractive index increment will strongly effect the molecular weight results. This is true for both cases, when SLS is used in batch mode, like in case of a goniometer system, or in flow mode like in case of a MALS Detector in GPC/SEC. This work explains and shows how dn/dc can be determined with extremely high accuracy with help of a specialized dn/dc Refractometer and Software.
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