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Achieving accurate temperature-independent flow measurement

Accurate measurement of flow is a critical parameter in liquid chromatography systems where retention times and peak shape are often directly affected by flow rate. In such systems, even slight variations in flow can degrade reproducibility and data integrity. In HPLC and GPC applications, temperature variations are inevitable either due to heated column compartments, pump motors, or pre-heated solvent. This raises the question of how solvent temperature may influence the accuracy of flow rate measurements. To investigate this, a study employing flow measurements was performed under controlled conditions using pre-heated solvent at two different temperatures.